Czech Hunter 127 Full

For visualising starch, leaves were excised from plants and placed in petri-dishes containing 100% ethanol (Roth, Karlsruhe, Germany) where they were allowed to de-stain overnight at room temperature. Once fully bleached, leaves were rinsed with water, and 6 mL iodine (Roth, Karlsruhe, Germany) was added to stain starch. After 30 min - 1 hr stained leaves were rinsed and excess stains was removed via suspension of leaves in water for 4-5 hrs at room temperature.

Abstract:p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis, and senescence. The human TP53 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure on the transactivation potential of full-length and N-terminal truncated p53α isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53α isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53α isoforms.Keywords: p53 protein; protein-DNA interaction; transactivation potential

Czech Hunter 127 Full

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One of a two-volume set, now divided between Cambridge and Prague (National Library of the Czech Republic, MS XXIII.C.120), this Pontifical contains the texts of liturgical offices and blessings performed by high-ranking churchmen. Long rectangular miniatures, with grounds of decorative scrollwork and ornate patterns, mark the main sections of the text. Most show bishops performing religious ceremonies with other clergy in attendance. The manuscript is decorated throughout with large historiated initials generally filled with subsidiary scenes relating to the ceremonies discussed in the text, but sometimes decorated with coats of arms or a standing figure of a bishop. The manuscript also features large ornamental initials with foliate motifs and interlace. The initials generally extend into full or partial bar borders inhabited by animals, birds, human figures and fanciful creatures. Small burnished gold initials mark lesser textual divisions. A wide variety of bas-de-page scenes, drawn and painted with consummate skill, offer additional visual interest. The rabbits, which are shown on many pages making mischief, are also found in other manuscripts associated with Renaud de Bar and may allude to the new fashion among the nobility for keeping warrens.

The Master of the Cambridge Pontifical of Renaud de Bar designed this page, but it was never painted. The partial bar border is decorated with foliate motifs. A standing bishop is depicted in the historiated initial A, and a mounted hunter with two hounds is shown in the bas-de-page. 2ff7e9595c